Protocol Writing

# Protocol Writing — Reproducible Lab Protocols & SOPs

## Overview
Write clear, reproducible experimental protocols and Standard Operating Procedures (SOPs) for any scientific discipline.

## Structure
1. **Title and Version** — Protocol name, version number, date, author
2. **Purpose** — What this protocol achieves and when to use it
3. **Safety** — Hazards, PPE requirements, waste disposal
4. **Materials** — Exact reagents (catalog numbers, lot numbers), equipment, consumables
5. **Preparation** — Buffer recipes, stock solutions, equipment setup
6. **Procedure** — Numbered steps with exact quantities, temperatures, times, speeds
7. **Quality Control** — Expected results, acceptance criteria, troubleshooting
8. **Data Recording** — What to record, where, in what format
9. **References** — Published methods this protocol is based on

## Key Principles
- **Exact quantities**: "Add 2.5 mL" not "add some"
- **Catalog numbers**: Every reagent identified by manufacturer and catalog number
- **Time precision**: "Incubate for 30 min at 37°C" not "incubate for a while"
- **Critical steps**: Mark steps where deviation causes failure
- **Troubleshooting table**: Common problems and solutions
- **Version control**: Track changes between protocol versions